This submission option is for genomic DNA (e.g. protein-coding gene, regulatory element), transcripts (e.g. mRNA, ncRNA), or small genomes (organelle, plasmid, and phage and other viral) from any organism. Artificial sequences (cloning/expression vector) as well as annotated or assembled third party sequences can also be submitted here.
mRNA submitters: Please try our new wizard for submitting Eukaryotic nuclear mRNA where you annotate CDS and other features in an enhanced web form.
You also have the option to upload
corresponding protein sequences for automated CDS annotation.
What You Should Expect
Prepare the following information:
General: your contact details, authors, publication, data release date
Original or third-party assembly/annotation
Set designation (if applicable) for multiple sequences of the same locus
Nucleotide sequences in FASTA or alignment format
Source metadata, such as: isolate, strain, collection date, country
Feature annotation, such as CDS (coding region), tRNA, ncRNA, gene
You may submit sequences in one of two formats:
1) FASTA, which is acceptable for one or more sequences. Please use the FASTA format that starts with a definition line, followed with a hard return and the sequence.
The simplest definition line requires the "> " symbol and a sequence_ID.
2) The alignment format, which is acceptable for multiple sequences from the same locus or same genomic region.
Accepted alignment formats include FASTA+GAP, Nexus, Phylip, and Clustal(w).
All sequence files must be in plain text using ASCII characters only. Use IUPAC codes for your sequences.
Source modifiers will be requested as part of submission and use a controlled vocabulary to describe how, when, and where you obtained your samples. You can also uniquely identify your samples from the same organism with source modifier such as isolate, clone, strain or specimen voucher.
You will be asked to provide values for certain source modifiers based on your organism information. Additional modifiers will be available to add.
Source modifiers can be provided through the web form or through a tab delimited table.
Prepare to annotate features on your sequence(s):
For simple annotation (e.g. same feature for all sequences), follow the web form's instructions
For complex annotation, prepare a tab-delimited, five-column feature table to upload
Provide feature intervals based on the sequence(s) you are submitting. For protein-coding sequences, annotate the coding regions (CDS) on your sequence(s), whether they are partial or complete.
If you submitted an alignment, you will have an option to 'Propagate features' from a single sequence (longest sequence recommended) to the other sequences in your submission. You will have the option to manually edit or remove features after propagation.
Not providing complete feature annotation will delay accession number assignment and processing.
Submit your sequence data on desktop. The desktop view allows you to easily:
NCBI provides multiple submission tools for different types of sequence data to be submitted.
BankIt can be used to submit most types of data (such as mRNA or genomic DNA; nuclear or organellar sequences; single sequences or sets of sequences; single genes or complete plasmids/organelles).
Submission Portal GenBank has specialized wizards designed to submit specific data types (Influenza, Norovirus, and Dengue; prokaryotic rRNA; organellar rRNA; eukaryotic rRNA-ITS; metazoan COX1), which streamlines the submission process.
Similarly, large genomes, transcriptomes, and most unassembled reads are submitted through alternate Submission Portal wizards (Genomes-WGS, TSA, and SRA, respectively).
For the latest submission options, begin by entering keywords describing the data you need to submit in the 'Search' box labeled 'What do you want to submit?' NCBI is in the process of improving the submission experience based on submitter feedback and activity. Some submission tools are being retired or combined to make it easier to submit and simplify requirements.
GenBank is the world's largest nucleotide archive containing sequences from all branches of life. The archive is a foundation for medical and biological discovery.