Submission Portal

Submit to the world's largest public repository of biological and scientific information


Use a streamlined submission process to submit the following data types (complete or partial sequences): SARS-CoV-2, Influenza A, B, or C, Norovirus, Dengue, prokaryotic ribosomal RNA (rRNA) and/or ribosomal intergenic spacer (IGS), eukaryotic nuclear rRNA and/or internal transcribed spacer (ITS), organelle rRNA and metazoan (multicellular animal) COX1 and eukaryotic nuclear mRNA.

What You Should Expect

This submission tool is for the specific data types indicated below.

  • SARS-CoV-2
  • Prokaryotic rRNA/IGS
  • Eukaryotic nuclear rRNA/ITS
  • Eukaryotic organelle rRNA
  • Metazoan (multicellular animal) COX1
  • Eukaryotic nuclear mRNA
  • Influenza, Norovirus or Dengue virus

Why are certain data types highlighted here?

Prepare the following information for your GenBank submission:

  1. General: contact details, authors, publication, data release date.
  2. Sequencing technology information
  3. FASTA-formatted sequences
  4. Organism name(s)
  5. Source metadata, such as: isolate, strain, collection date, country
  6. Feature Annotation: Features are automatically annotated for specific submissions, including SARS-CoV-2, Influenza, Dengue virus, Norovirus, metazoan COX1, rRNA, and rRNA-ITS. For eukaryotic mRNA submissions, we ask you to annotate CDS features. You have the flexibility to provide CDS annotations through a web form or by submitting the corresponding protein sequences. Additionally, you can use a web form to annotate UTR features. If you prefer to use a feature table for annotation, please use BankIt instead.

Prepare your sequence(s) in the FASTA format that starts with a definition line, followed with a hard return and the sequence. The simplest definition line requires the “> “symbol and a sequence_ID.



Use only ASCII characters for your definition line and IUPAC codes for your sequences. Upload a FASTA file as a plain-text file (prepared with a text editor). The file may have one or more sequences.

Source modifiers will be requested as part of submission and use a controlled vocabulary to describe how, when, and where you obtained your samples. You can also uniquely identify your samples from the same organism with source modifier such as isolate, clone, strain or specimen voucher.

You will be asked to provide values for certain source modifiers based on your submission type. Additional modifiers will be available to add.

Your submission will be automatically screened for common problems such as vector or adaptor contamination, chimeras, source problems, and various sequence errors. The types of checks performed will depend on the submission type.

An error report with explanations of the errors will be provided, if applicable. You must fix the errors before accession numbers are assigned. After your errors are corrected, your submission will be reviewed by NCBI for further processing and accession assignment.

Submit your sequence data on desktop. The desktop view allows you to easily:

  • Enter your information
  • Enter or upload metadata
  • Upload large source files
  • Review your submission

Email me a link to get started


GenBank FAQ

  • NCBI provides multiple submission tools for different types of sequence data. BankIt can be used to submit most types of data (such as genomic DNA; nuclear or organellar sequences; single genes or complete plasmids/organelles). Submission Portal GenBank has specialized wizards designed to submit specific data types (SARS CoV-2, Influenza, Norovirus, and Dengue; prokaryotic rRNA; organellar rRNA; eukaryotic rRNA-ITS; metazoan COX1; eukaryotic nuclear mRNA), which streamlines the submission process. Work is in progress to accommodate more data types in Submission Portal GenBank as we work towards consolidating and enhancing our submission tools. Similarly, large genomes, transcriptomes, and most unassembled reads are submitted through alternative Submission Portal wizards (Genomes-WGS, TSA, and SRA, respectively). If you have questions about which tool is appropriate for your data type, please contact

  • For the latest submission options, begin by entering keywords describing the data you need to submit in the 'Search' box labeled 'What do you want to submit?' NCBI is in the process of improving the submission experience based on submitter feedback and activity. Some submission tools are being retired or combined to make it easier to submit and simplify requirements.


GenBank is the world's largest nucleotide archive containing sequences from all branches of life. The archive is a foundation for medical and biological discovery.

  • Submit assembled SARS-CoV-2, Influenza, Norovirus, Dengue virus, rRNA, rRNA-ITS, metazoan COX1, Eukaryotic nuclear mRNA sequences.

  • Submit genomic DNA, organelle, ncRNA, plasmids, other viruses, phages, mRNA, synthetic constructs.

  • Submit assembled eukaryotic and prokaryotic genomes (WGS or Complete).

Sequence Read Archive (SRA)

SRA is the largest publicly-available repository of high throughput sequencing data. The archive accepts data from all branches of life as well as metagenomic and environmental surveys.

Other Tools

  • TSA

    Submit computationally assembled, transcribed RNA sequences after submitting unassembled reads to SRA. Learn more

  • GEO

    Submit RNA-seq, ChIP-seq, and other types of gene expression and epigenomics datasets. Learn more

  • BioProject & BioSample

    Choose a tool above if submitting sequence data. Learn more

Medical Genetics & Variation Tools

Submit clinical data, small & large human genomics variants, and genotype & phenotype data.

Other Resources