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GenBank and Sequence Read Archive (SRA) both accept large-scale targeted sequencing projects (>2,500 sequences)
for either a single gene locus from multiple organisms or multiple conserved elements derived from a single organism.
What You Should Expect
A Targeted Locus Study (TLS) is a large-scale targeted sequencing project (>2,500 sequences)
for either a single gene locus from multiple organisms or multiple conserved elements derived from a single organism.
All sequences included within a single TLS submission are linked within a single BioProject and include BioSamples
for each isolation source included in the submission.
The process for TLS submission is different depending on whether your loci are rRNA or not.
If the locus in in your TLS is rRNA, and data is assembled, you may submit to both GenBank and Sequence Read Archive (SRA).
Begin with the SRA submission and create your BioProject and BioSample during submission.
If the locus is anything other than rRNA, begin by submitting your reads to SRA.
You will then need to prepare an ASN.1 (.sqn) file using tbls2asn.
Depending on whether you submitted TLS data for rRNA or not, you will complete the submission process
by following the steps for Sequence Read Archive (SRA) and GenBank submission.
Note that once the tbl2asn step is completed for non-rRNA, you should email the file to gb-sub@ncbi.nlm.nih.gov.
Submit your sequence data on desktop. The desktop view allows you to easily:
GenBank is the world's largest nucleotide archive containing sequences from all branches of life. The archive is a foundation for medical and biological discovery.
Submit genomic DNA, organelle, ncRNA, plasmids, other viruses, phages, other mRNA, synthetic constructs.
Submit assembled prokaryotic and eukaryotic genomes.
Sequence Read Archive (SRA)
SRA is the largest publicly-available repository of high throughput sequencing data. The archive accepts data from all branches of life as well as metagenomic and environmental surveys.
Submit unassembled, high throughput sequencing reads