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Targeted Locus Study (TLS)

GenBank and Sequence Read Archive (SRA) both accept large-scale targeted sequencing projects (>2,500 sequences) for either a single gene locus from multiple organisms or multiple conserved elements derived from a single organism.

What You Should Expect

A Targeted Locus Study (TLS) is a large-scale targeted sequencing project (>2,500 sequences) for either a single gene locus from multiple organisms or multiple conserved elements derived from a single organism.

All sequences included within a single TLS submission are linked within a single BioProject and include BioSamples for each isolation source included in the submission.

The process for TLS submission is different depending on whether your loci are rRNA or not.

  • If the locus in in your TLS is rRNA, and data is assembled, you may submit to both GenBank and Sequence Read Archive (SRA). Begin with the SRA submission and create your BioProject and BioSample during submission.
  • If the locus is anything other than rRNA, begin by submitting your reads to SRA. You will then need to prepare an ASN.1 (.sqn) file using tbls2asn.

To complete the submission, email the file to gb-sub@ncbi.nlm.nih.gov.

Depending on whether you submitted TLS data for rRNA or not, you will complete the submission process by following the steps for Sequence Read Archive (SRA) and GenBank submission.

Note that once the tbl2asn step is completed for non-rRNA, you should email the file to gb-sub@ncbi.nlm.nih.gov.

Submit your sequence data on desktop. The desktop view allows you to easily:

  • Enter your information
  • Enter or upload metadata
  • Upload large source files
  • Review your submission

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Targeted Locus Study (TLS) FAQ

GenBank

GenBank is the world's largest nucleotide archive containing sequences from all branches of life. The archive is a foundation for medical and biological discovery.

  • Submit assembled ribosomal RNA (rRNA), rRNA-ITS, SARS-CoV-2, Influenza, Norovirus or metazoan COX1 sequences.

  • Submit mRNA, genomic DNA, organelle, ncRNA, plasmids, other viruses, phages, synthetic constructs.

  • Submit assembled eukaryotic and prokaryotic genomes (WGS or Complete).

Sequence Read Archive (SRA)

SRA is the largest publicly-available repository of high throughput sequencing data. The archive accepts data from all branches of life as well as metagenomic and environmental surveys.

Other Tools

  • TSA

    Submit computationally assembled, transcribed RNA sequences after submitting unassembled reads to SRA. Learn more

  • GEO

    Submit RNA-seq, ChIP-seq, and other types of gene expression and epigenomics datasets. Learn more

  • BioProject & BioSample

    Choose a tool above if submitting sequence data. Learn more

Medical Genetics & Variation Tools

Submit clinical data, small & large human genomics variants, and genotype & phenotype data.

Other Resources